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基本信息

質粒圖譜和多克隆位點信息

注意：

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如要使用本公司保存的母源載體時，本公司不能保證所有母源載體完全符合客戶預期，也不承擔由此產生的任何責任。

載體描述

pCS2+載體能夠在多種細胞中進行高水平的瞬表達。表達細胞：原核細胞、哺乳動物、非洲爪蟾、鳥類、斑馬魚。pCS2+載體同時也能進行體外轉錄和翻譯。本載體在pCS2+載體基礎上改造而來，增加了Flag蛋白標簽，便于檢測和分離。

載體序列

LOCUS       Exported                4110 bp ds-DNA     circular SYN 20-APR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pCS(+)-Flag

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4110)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Saturday, Apr 20, 2019 from SnapGene Viewer 4.3.6

FEATURES             Location/Qualifiers

     source          1..4110

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        35..53

                     /label=SP6 promoter

                     /note="promoter for bacteriophage SP6 RNA polymerase"

     primer_bind     35..53

                     /label=SP6 fwd

     misc_feature    79..99

                     /label=MCS1

     CDS             103..126

                     /codon_start=1

                     /product="FLAG(R) epitope tag, followed by an enterokinase

                     cleavage site"

                     /label=FLAG

                     /translation="DYKDDDDK"

     promoter        complement(136..154)

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     polyA_signal    159..293

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     primer_bind     complement(183..202)

                     /label=EBV rev

     primer_bind     237..256

                     /label=SV40 polyA rev

     misc_feature    360..393

                     /label=MCS2

     primer_bind     complement(405..424)

                     /label=T3 fwd

     promoter        complement(406..424)

                     /label=T3 promoter

                     /note="promoter for bacteriophage T3 RNA polymerase"

     primer_bind     complement(445..461)

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar

                     variants"

     protein_bind    469..485

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to

                     inhibit transcription in E. coli. This inhibition can be

                     relieved by adding lactose or

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     promoter        complement(493..523)

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    538..559

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence

                     of cAMP."

     rep_origin      complement(847..1435)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     CDS             complement(1606..2466)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(2467..2571)

                     /gene="bla"

                     /label=AmpR promoter

     rep_origin      complement(2597..3052)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow

                     indicates direction of (+) strand synthesis"

     promoter        3126..4110

                     /label=CMV IE94 promoter

                     /note="enhancer/promoter region of simian cytomegalovirus

                     major immediate early transcription unit IE94"

ORIGIN

        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag

       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca tggattacaa ggatgacgac

      121 gataagtaat ctagaactat agtgagtcgt attacgtaga tccagacatg ataagataca

      181 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa

      241 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca

      301 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaattcg

      361 cggccgcggc gccaatgcat tgggcccggt acccagcttt tgttcccttt agtgagggtt

      421 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct

      481 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg

      541 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct

      601 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg

      661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc

      721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg

      781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct

      841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca

      901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct

      961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc

     1021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt

     1081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc

     1141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc

     1201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg

     1261 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc

     1321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag

     1381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga

     1441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat

     1501 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag

     1561 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat

     1621 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc

     1681 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat

     1741 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag

     1801 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg

     1861 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc

     1921 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca

     1981 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg

     2041 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc

     2101 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta

     2161 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc

     2221 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg

     2281 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc

     2341 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc

     2401 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat

     2461 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag

     2521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc

     2581 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt

     2641 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca

     2701 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta

     2761 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta

     2821 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg

     2881 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga

     2941 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg

     3001 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg

     3061 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc

     3121 cagtcgacca tagccaattc aatatggcgt atatggactc atgccaattc aatatggtgg

     3181 atctggacct gtgccaattc aatatggcgt atatggactc gtgccaattc aatatggtgg

     3241 atctggaccc cagccaattc aatatggcgg acttggcacc atgccaattc aatatggcgg

     3301 acttggcact gtgccaactg gggaggggtc tacttggcac ggtgccaagt ttgaggaggg

     3361 gtcttggccc tgtgccaagt ccgccatatt gaattggcat ggtgccaata atggcggcca

     3421 tattggctat atgccaggat caatatatag gcaatatcca atatggccct atgccaatat

     3481 ggctattggc caggttcaat actatgtatt ggccctatgc catatagtat tccatatatg

     3541 ggttttccta ttgacgtaga tagcccctcc caatgggcgg tcccatatac catatatggg

     3601 gcttcctaat accgcccata gccactcccc cattgacgtc aatggtctct atatatggtc

     3661 tttcctattg acgtcatatg ggcggtccta ttgacgtata tggcgcctcc cccattgacg

     3721 tcaattacgg taaatggccc gcctggctca atgcccattg acgtcaatag gaccacccac

     3781 cattgacgtc aatgggatgg ctcattgccc attcatatcc gttctcacgc cccctattga

     3841 cgtcaatgac ggtaaatggc ccacttggca gtacatcaat atctattaat agtaacttgg

     3901 caagtacatt actattggaa ggacgccagg gtacattggc agtactccca ttgacgtcaa

     3961 tggcggtaaa tggcccgcga tggctgccaa gtacatcccc attgacgtca atggggaggg

     4021 gcaatgacgc aaatgggcgt tccattgacg taaatgggcg gtaggcgtgc ctaatgggag

     4081 gtctatataa gcaatgctcg tttagggaac

//