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基本信息

質粒圖譜和多克隆位點信息

載體描述

pCS2+載體能夠在多種細胞中進行高水平的瞬表達。表達細胞：原核細胞、哺乳動物、非洲爪蟾、鳥類、斑馬魚。pCS2+載體同時也能進行體外轉錄和翻譯。本載體在pCS2+載體基礎上改造而來，增加了HA蛋白標簽，便于檢測。

 載體序列

      LOCUS       Exported                4113 bp ds-DNA     circular SYN 19-APR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pCS2(+)-HA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4113)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Friday, Apr 19, 2019 from SnapGene Viewer 4.3.6

FEATURES             Location/Qualifiers

     source          1..4113

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        35..53

                     /label=SP6 promoter

                     /note="promoter for bacteriophage SP6 RNA polymerase"

     primer_bind     35..53

                     /label=SP6 fwd

     misc_feature    79..99

                     /label=MCS1

                     /note="Multiple cloning site"

     CDS             103..129

                     /codon_start=1

                     /product="HA (human influenza hemagglutinin) epitope tag"

                     /label=HA

                     /translation="YPYDVPDYA"

     promoter        complement(139..157)

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     polyA_signal    162..296

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     primer_bind     complement(186..205)

                     /label=EBV rev

     primer_bind     240..259

                     /label=SV40 polyA rev

     misc_feature    297..396

                     /label=MCS2

     primer_bind     complement(408..427)

                     /label=T3 fwd

     promoter        complement(409..427)

                     /label=T3 promoter

                     /note="promoter for bacteriophage T3 RNA polymerase"

     primer_bind     complement(448..464)

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar

                     variants"

     protein_bind    472..488

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to

                     inhibit transcription in E. coli. This inhibition can be

                     relieved by adding lactose or

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     promoter        complement(496..526)

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    541..562

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence

                     of cAMP."

     rep_origin      complement(850..1438)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     CDS             complement(1609..2469)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(2470..2574)

                     /gene="bla"

                     /label=AmpR promoter

     rep_origin      complement(2600..3055)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow

                     indicates direction of (+) strand synthesis"

     promoter        3129..4113

                     /label=CMV IE94 promoter

                     /note="enhancer/promoter region of simian cytomegalovirus

                     major immediate early transcription unit IE94"

ORIGIN

        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag

       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca tgtacccata cgacgtccca

      121 gactacgctt aatctagaac tatagtgagt cgtattacgt agatccagac atgataagat

      181 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg

      241 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca

      301 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaat

      361 tcgcggccgc ggcgccaatg cattgggccc ggtacccagc ttttgttccc tttagtgagg

      421 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc

      481 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta

      541 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa

      601 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat

      661 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg

      721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc

      781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt

      841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag

      901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc

      961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc

     1021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt

     1081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt

     1141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc

     1201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa

     1261 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa

     1321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg

     1381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga

     1441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg

     1501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg

     1561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt

     1621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact

     1681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat

     1741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg

     1801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg

     1861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat

     1921 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc

     1981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt

     2041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc

     2101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga

     2161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc

     2221 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa

     2281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta

     2341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg

     2401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg

     2461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat

     2521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt

     2581 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat

     2641 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa

     2701 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta

     2761 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca

     2821 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat

     2881 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg

     2941 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc

     3001 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat

     3061 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta

     3121 cgccagtcga ccatagccaa ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg

     3181 tggatctgga cctgtgccaa ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg

     3241 tggatctgga ccccagccaa ttcaatatgg cggacttggc accatgccaa ttcaatatgg

     3301 cggacttggc actgtgccaa ctggggaggg gtctacttgg cacggtgcca agtttgagga

     3361 ggggtcttgg ccctgtgcca agtccgccat attgaattgg catggtgcca ataatggcgg

     3421 ccatattggc tatatgccag gatcaatata taggcaatat ccaatatggc cctatgccaa

     3481 tatggctatt ggccaggttc aatactatgt attggcccta tgccatatag tattccatat

     3541 atgggttttc ctattgacgt agatagcccc tcccaatggg cggtcccata taccatatat

     3601 ggggcttcct aataccgccc atagccactc ccccattgac gtcaatggtc tctatatatg

     3661 gtctttccta ttgacgtcat atgggcggtc ctattgacgt atatggcgcc tcccccattg

     3721 acgtcaatta cggtaaatgg cccgcctggc tcaatgccca ttgacgtcaa taggaccacc

     3781 caccattgac gtcaatggga tggctcattg cccattcata tccgttctca cgccccctat

     3841 tgacgtcaat gacggtaaat ggcccacttg gcagtacatc aatatctatt aatagtaact

     3901 tggcaagtac attactattg gaaggacgcc agggtacatt ggcagtactc ccattgacgt

     3961 caatggcggt aaatggcccg cgatggctgc caagtacatc cccattgacg tcaatgggga

     4021 ggggcaatga cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg

     4081 gaggtctata taagcaatgc tcgtttaggg aac

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