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基本信息

質粒圖譜和多克隆位點信息

注意：

客戶在我公司選擇構建載體服務項目時，請自行提供母源載體。

如要使用本公司保存的母源載體時，本公司不能保證所有母源載體完全符合客戶預期，也不承擔由此產生的任何責任。

載體描述

本載體的特征是允許在大腸桿菌中進行高拷貝復制和在哺乳動物細胞中高水平的瞬時表達蛋白。

載體包含下列關鍵元素：

?人巨細胞病毒（CMV）立即早期啟動子，使得本載體可以在大部分的哺乳動物細胞中進行高水平基因表達。

?牛生長激素（BGH）多聚腺苷酸化信號能夠保證高效的轉錄終止和mRNA的poly A結構。

?在大腸桿菌中是用卡那抗性進行載體篩選。

?質粒pVAX1/lacZ是用來作為細胞系目的基因轉染和表達的陽性對照。

載體序列

LOCUS       Exported                2999 bp ds-DNA     circular SYN 30-MAR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pVAX1

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 2999)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Saturday, Mar 30, 2019 from SnapGene Viewer 4.3.4

FEATURES             Location/Qualifiers

     source          1..2999

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        36..415

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        416..619

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early

                     promoter"

     primer_bind     570..590

                     /label=CMV F

     promoter        664..682

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     misc_feature    696..735

                     /label=MCS

                     /note="multiple cloning site"

     primer_bind     complement(823..840)

                     /label=BGH Reverse

     polyA_signal    829..1053

                     /label=bGH poly(A) signal

                     /note="bovine growth hormone polyadenylation signal"

     CDS             1226..2020

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     rep_origin      2346..2934

                     /direction=RIGHT

                     /label=pUC ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

ORIGIN

        1 gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta

       61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata

      121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat

      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga

      241 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc

      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt

      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat

      421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag

      481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc

      541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga

      601 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga

      661 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt

      721 accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc

      781 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta

      841 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca

      901 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc

      961 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata

     1021 gcaggcatgc tggggatgcg gtgggctcta tggcttctac tgggcggttt tatggacagc

     1081 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt

     1141 aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca

     1201 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc

     1261 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc

     1321 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga

     1381 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac

     1441 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct

     1501 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa

     1561 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc

     1621 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct

     1681 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc

     1741 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg

     1801 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct

     1861 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct

     1921 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca

     1981 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attattaacg cttacaattt

     2041 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacaggtggc

     2101 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat

     2161 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatagca cgtgctaaaa

     2221 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa

     2281 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga

     2341 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg

     2401 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact

     2461 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac

     2521 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg

     2581 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg

     2641 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga

     2701 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc

     2761 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg

     2821 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc

     2881 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc

     2941 agcaacgcgg cctttttacg gttcctgggc ttttgctggc cttttgctca catgttctt

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