請在線咨詢或直接聯系18986294316/17720555249

必騰預構載體定制最高200元/個

基本信息

質粒圖譜和多克隆位點信息

注意：

客戶在我公司選擇構建載體服務項目時，請自行提供母源載體。

如要使用本公司保存的母源載體時，本公司不能保證所有母源載體完全符合客戶預期，也不承擔由此產生的任何責任。

載體描述

pFastBac1系列載體，昆蟲表達載體，融合表達EGFP。

 載體序列

LOCUS       Exported                4776 bp ds-DNA     circular SYN 30-MAR-2019

LOCUS       Exported                5597 bp ds-DNA     circular SYN 30-MAR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pFastBac1-eGFP

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 5597)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Saturday, Mar 30, 2019 from SnapGene Viewer 4.3.4

FEATURES             Location/Qualifiers

     source          1..5597

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     rep_origin      2..457

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow

                     indicates direction of (+) strand synthesis"

     promoter        484..588

                     /gene="bla"

                     /label=AmpR promoter

     CDS             589..1449

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      1620..2208

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     mobile_element  2511..2735

                     /mobile_element_type="transposon:Tn7"

                     /note="mini-Tn7 element (right end of the Tn7 transposon)"

     CDS             complement(2802..3335)

                     /codon_start=1

                     /gene="aacC1"

                     /product="gentamycin acetyltransferase"

                     /label=GmR

                     /note="confers resistance to gentamycin"

                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD

                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS

                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR

                     EEVMHFDIDPSTAT"

     promoter        complement(3524..3552)

                     /gene="intI1 (promoter lies within the coding sequence)"

                     /label=Pc promoter

                     /note="class 1 integron promoter"

     promoter        3904..3995

                     /gene="polh from Autographa californica"

                     /label=polyhedrin promoter

                     /note="promoter for the baculovirus polyhedrin gene"

     primer_bind     3995..4014

                     /label=pFastBac-F

     CDS             4062..4079

                     /codon_start=1

                     /product="6xHis affinity tag"

                     /label=6xHis

                     /translation="HHHHHH"

     CDS             4101..4121

                     /codon_start=1

                     /product="tobacco etch virus (TEV) protease recognition and

                     cleavage site"

                     /label=TEV site

                     /translation="ENLYFQG"

     misc_feature    4122..4139

                     /label=5' MCS

     CDS             4140..4856

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     CDS             4857..4874

                     /codon_start=1

                     /product="thrombin recognition and cleavage site"

                     /label=thrombin site

                     /translation="LVPRGS"

     misc_feature    4875..4908

                     /label=3'MCS

     primer_bind     complement(4989..5007)

                     /label=pFastBac-R

     polyA_signal    5087..5221

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     mobile_element  5250..5415

                     /mobile_element_type="transposon:Tn7"

                     /note="mini-Tn7 element (left end of the Tn7 transposon)"

ORIGIN

        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc

       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc

      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt

      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg

      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt

      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta

      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt

      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat

      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg

      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa

      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac

      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac

      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt

      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc

      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca

      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc

      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag

     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa

     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg

     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa

     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg

     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt

     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt

     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag

     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat

     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct

     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct

     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca

     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc

     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc

     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct

     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag

     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc

     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg

     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag

     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt

     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac

     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg

     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc

     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg

     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct

     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga

     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag

     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt

     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga

     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac

     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg

     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg

     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca

     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact

     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc

     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta

     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct

     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg

     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg

     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca

     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa

     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa

     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca

     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac

     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc

     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg

     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt

     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt

     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa

     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt

     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca

     4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg

     4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatggga tctgctagca

     4141 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg

     4201 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg

     4261 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc

     4321 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc

     4381 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct

     4441 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg

     4501 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca

     4561 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg

     4621 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg

     4681 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact

     4741 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc

     4801 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagctgg

     4861 ttccgcgtgg atccgaattc aaaggcctac gtcgacgagc tcactagtcg cggccgcttt

     4921 cgaatctaga gcctgcagtc tcgaggcatg cggtaccaag cttgtcgaga agtactagag

     4981 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca

     5041 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc

     5101 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt

     5161 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat

     5221 ctgatcactg atatcgccta ggagatccga accagataag tgaaatctag ttccaaacta

     5281 ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc catctatttt

     5341 gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta

     5401 ttttgtccgc ccacagcggg gcatttttct tcctgttatg tttttaatca aacatcctgc

     5461 caactccatg tgacaaaccg tcatcttcgg ctactttttc tctgtcacag aatgaaaatt

     5521 tttctgtcat ctcttcgtta ttaatgtttg taattgactg aatatcaacg cttatttgca

     5581 gcctgaatgg cgaatgg

//